Figure S4. TLR2 exerts an inhibitory effect on Th1 responses induced by IFN-γ. Isolated lymph node cells from mice 10 d after injection with YF-17D or mock infection were plated at 5 X 10⁵ cells/well in 96-well plates and cultured in the presence of a combination YF-17D CD4 and CD8 T cell epitopes. The CD4⁺ T cell epitope is an I-Ab-restricted epitope from the viral envelope protein (residues 231-247 from the envelope protein, LVEFEPPHAATIRVL), whereas the CD8⁺ T cell epitopes are comprised of one subdominant H-2Db-restricted epitope (residues 1-15 from the E1 envelope protein, AHCIGITDRDFIEGV), and another H-2Kb-restricted dominant epitope mapped to the NS3 protein (residues 261-275 from the NS3 protein, VIDAMCHATLTYRML) (van der Most, R.G., L.E. Harrington, V. Giuggio, P.L. Mahar, and R. Ahmed. 2002. Virology. 296:117-124). Supernatants were collected 4 d later and analyzed by ELSIA for IFN- γ. Data are representative of three independent experiments, with three to four mice per group in each experiment.