Impaired response to Listeria in H2-M3-deficient mice reveals a nonredundant role of MHC class Ib-specific T cells in host defense -- Data Supplement - JEM--Xu et al. -- The Journal of Experimental Medicine

Table S1. Frequency of CD8 subpopulations in H2-M3–deficient and WT mice

		Percentage of Lymphocytes
Tissue	Phenotype	WT	M3^-/-
Thymus	CD69⁺ Ly6C⁺ CD122⁺ CD44⁺	13.7 ± 0.1 0.4 ± 0.0 0.5 ± 0.2 1.0 ± 0.2	14.5 ± 0.8 0.4 ± 0.1 0.3 ± 0.1 1.0 ± 0.2
PBMCs	CD69⁺ Ly6C⁺ CD122⁺ CD44⁺	3.0 ± 0.8 47.7 ± 1.6 10.1 ± 2.6 38.9 ± 3.2	3.3 ± 0.8 43.5 ± 2.2 10.6 ± 2.4 33.3 ± 2.2
Spleen	CD69⁺ Ly6C⁺ CD122⁺ CD44⁺	10.6 ± 0.7 46.5 ± 1.2 14.2 ± 3.3 41.6 ± 1.7	10.0 ± 0.6 41.8 ± 2.7 12.2 ± 2.3 37.4 ± 1.2	(P = 0.06)
Mesenteric Lymph Nodes	CD69⁺ Ly6C⁺ CD122⁺ CD44⁺	13.6 ± 2.0 44.3 ± 1.9 9.5 ± 2.5 33.8 ± 3.0	13.5 ± 1.1 39.1 ± 2.6 7.4 ± 1.6 29.1 ± 1.8	(P = 0.05)
Liver	CD69⁺ Ly6C⁺ CD122⁺ CD44⁺	43.2 ± 2.7 35.5 ± 2.5 27.7 ± 2.0 37.7 ± 1.9	43.7 ± 2.9 34.1 ± 5.5 28.5 ± 1.6 34.7 ± 2.2
Values represent mean (± SEM) results obtained from eight mice (3–4 months old) per group. Lymphocytes from various organs were stained with CyChrome–anti-CD8 and antibodies against CD44, CD69, CD122, or Ly6C conjugated with either FITC or PE.