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Figure S4. Both CD8^- and CD8⁺ DCs from uninfected mice induced proliferation, IL-2, IFN-γ, IL-4, and IL-10 production in malaria-specific TCR Tg T cells. (A) Sorted CD11c⁺CD8⁺ (open squares) and CD8^- (closed circles) DCs from uninfected mice were cultured in the presence of 10⁵ enriched CD4⁺ B5 T cells for 3 d and were pulsed with [³H]thymidine as described. DCs from uninfected mice were incubated with 1 µM peptide or 30:1 schizonts/DC. (B) 5 x 10 ⁴ sorted CD8⁺ (white bars) and CD8^- (black bars) splenic DCs from uninfected mice were cultured in the presence of 10⁵ enriched CD4⁺ B5 T cells and 30:1 schizonts/DC or 1 µM synthetic B5 peptide. After 6 d of culture, the cells were washed, counted, and cultured at 10⁵ cell/well in 96-well plates in the presence of cross-linked anti-CD3 and 2 µg/ml anti-CD28 antibodies. IL-2 content was determined in the supernatant by ELISA after 24 h of culture, and IFN-γ, IL-4, and IL-10 content was determined after 48 h. Graphs are of the mean values with SEM (error bars) of triplicate samples. *, P = 0.05-0.01 (Student’s t test). The data are from a representative experiment of three performed.