T-bet negatively regulates autoimmune myocarditis by suppressing local production of interleukin 17 -- Data Supplement - JEM--Rangachari et al. -- The Journal of Experimental Medicine

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Figure S3. Peripheral T-bet^-/- CD8⁺ T cells function normally. (A) Cytotoxicity assay of T-bet^+/+ versus T-bet^-/- CD8⁺ T cells. Allo-primed and restimulated CD8⁺ T cells from either T-bet^+/+ or T-bet^-/- mice were used at 40:1, 13:1, or 4:1 ratios on calcein-labeled EL-4 target cells. Results are expressed as mean of percent-specific lysis from quadruplicates ± SD. (B) T-bet^+/+ and T-bet^-/- peripheral CD8β⁺ cells were stimulated with soluble anti-CD3 plus γ-irradiated syngeneic splenocytes (APCs), or with plate-bound anti-CD3/anti-CD28. IFN-γ production was assessed using intracellular cytokine staining. Representative data from three mice each is shown. (C) Whole T-bet^+/+ or T-bet^-/- splenocytes from immunized mice were restimulated with MyHC-α or anti-CD3/anti-CD28. IFN-γ production was measured from supernatants at 48 h.