Table S1. Ligand potencies (EC50 values) determined in CD69 upregulation assays
Cell type | Ligands | ||||||
4L EC50 (M) | 4P EC50 (M) | 4V EC50 (M) | 4A EC50 (M) | 4S EC50 (M) | 4N EC50 (M) | 4H EC50 (M) | |
DP thymocyte | 8.4 X 10-13 | 3.0 X 10-12 | 6.4 X 10-12 | 4.5 X 10-11 | 5.0 X 10-11 | 8.8 X 10-10 | 1.2 X 10-7 |
SP thymocyte | 5.8 X 10-13 | 2.4 X 10-12 | 4.1 X 10-12 | 9.0 X 10-11 | 7.3 X 10-11 | 1.8 X 10-7 | 5.5 X 10-6 a |
LN T cell | 8.8 X 10-13 | 5.6 X 10-13 | 6.1 X 10-12 | 2.7 X 10-10 | 1.7 X 10-10 | 1.1 X 10-7 | 7.1 X 10-6 a |
Mean values of at least three independent experiments are shown. Values corrected for slight differences in peptide–MHC binding. aLess than 50% of all cells responded. | |||||||