Table S2. Half-lives (t1/2 [s]) of pMHC monomer binding to T1 thymocytes

Cell type

Ligands (pMHC monomers)

4P-Kd (0°C)
t1/2 (s)
4A-Kd (0°C)
t 1/2 (s)
4N-Kd (0°C)
t 1/2 (s)
4P-Kd (37°C)
t 1/2 (s)
DP thymocyte
1,380 ± 240
28 ± 8
<15
39 ± 10
SP thymocyte
1,440 ± 300
30 ± 10
<15
41 ± 18
Mean values and SEMs of at least three independent experiments are shown.