[View Larger Version of this Image]

Figure S1. Monocytes accumulate in the myocardium after MI. (A) Cell suspensions from C57BL/6 mouse peripheral blood, spleen, healthy heart (Pre), or infarcted heart 1 d after MI were stained with anti-CD11b, -CD90, -B220, -CD49b, -NK1.1, -Ly-6G, -Ly-6C, -F4/80, -I-A^b, and -CD11c mAbs. Representative dot plots depict monocytes/macrophages/dendritic cells identified as CD11b^hi (CD90/B220/CD49b/NK1.1/Ly-6G)^lo. These cells were further divided into Ly-6C^hi monocytes (Ly-6C^hi [F4/80/I-A^b/CD11c]^lo), Ly-6C^lo monocytes (Ly-6C^lo [F4/80/I-A^b/CD11c]^lo), and macrophages/dendritic cells (Ly-6C^lo [F4/80/I-A^b/CD11c]^hi). (B) Representative cytospin preparations depicting Ly-6C^hi monocytes, Ly-6C^lo monocytes, and macrophages/dendritic cells after cell sorting of splenic Ly-6C^hi (F4/80/I-A^b/CD11c)^lo, Ly-6C^lo (F4/80/I-A^b/CD11c)^lo, and Ly-6C^lo (F4/80/I-A^b/CD11c)^hi cells, respectively. Bars, 10 μm. (C) Cell suspensions from C57BL/6 mouse healthy heart (Pre) or infarcted heart 1 or 4 d after MI. Representative dot plots depict CD11b^hi (CD90/B220/CD49b/NK1.1/Ly-6G)^lo and show F4/80, I-A^b, or CD11c expression separately for Ly-6C^hi and -6C^lo cells.