Conditional inactivation of Fbxw7 impairs cell-cycle exit during T cell differentiation and results in lymphomatogenesis -- Data Supplement - JEM--Onoyama et al. -- The Journal of Experimental Medicine

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Figure S4. Tumorigenicity of lymphoma cells in nude mice. (A) 3 wk after intraperitoneal injection of lymphoma cells, various patterns of infiltration were observed. (left) Abdominal distension caused by ascites. (top right) Subcutaneous tumors (arrowhead). (bottom right) Abdominal lymphadenopathy (arrowheads). (B) Surface expression of CD4 and CD8 on thymic lymphoma cells transplanted into a nude mouse (top) and on the cells of a tumor that developed in the nude mouse (bottom). (C) Southern blot analysis of genomic DNA from normal thymocytes, from the thymic lymphoma cells transplanted into a nude mouse, from two tumors that developed from the transplanted cells in the nude mouse, and from the tail of a wild-type mouse. The DNA was digested with BamHI and probed with a 2.1-kb EcoRI fragment of the TCR-Jβ2 locus. The position of the germline fragment is indicated.