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Figure S2. Expression of cytokine RNA in the colon. (A) Total RNA was prepared from wild-type (+/+) or Cyld^-/- mice (males 8 wk of age) and subjected to semiquantitative RT-PCR analyses to detect the mRNA of IL-10 and Gapdh. (B) Total RNA was prepared from optimal cutting temperature-embedded frozen colons derived from RAG1 knockout mice adoptively transferred with T cells isolated from wild-type (+/+) or Cyld^-/- mice (same tissues used in Fig. 3). Semiquantitative RT-PCR assays were performed to analyze the RNA expression of the indicated genes. The sizes of PCR products are indicated.