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Figure S4. CYLD is not important for turning off the IKK activation signal in Jurkat T cells. (A) Jurkat-pSUPER and -shCYLD cells were stimulated with either 20 ng/ml TNF-α or 50 ng/ml PMA plus 1 µM ionomycin for the indicated times followed by IKKβ kinase assays and IB. (B) Lack of induction of CYLD along with mitogen stimulation. Jurkat-sSUPER and -shCYLD cells were stimulated with PMA plus ionomycin for the indicated times followed by IB to detect CYLD and tubulin. A nonspecific band is labeled ns.