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Figure S1. CD4⁺CD25⁺CD45RO⁺IL-7Rα^high cells secrete inflammatory cytokines. 2 x 10⁶ cryopreserved blood mononuclear cells from liver and kidney transplant recipients were prestained with anti-IL-7Rα and anti-CD25 antibodies and stimulated overnight with soluble anti-CD3 plus anti-CD28 antibodies in complete medium containing GolgiPlug and GolgiStop. At the end of the stimulation period, the cells were stained with CD4 and CD45RO antibodies, and permeabilized and stained with IFN-γ or TNF-α and IL-2. The CD4⁺CD25⁺CD45RO⁺IL-7Rα^high cell population contained a significant percentage of cells secreting both IFN-γ and TNF-α. Data are expressed as the mean ± SE.