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Figure S2. Thymocyte positive and negative selection in Runx1^F/F;Cd4-cre mice. (A) Differentiation of CD4SP and CD8SP thymocytes in Runx1^F/F;Cd4-cre mice in the presence of DO11.10 or HY TCR transgenes. CD4 and CD8 expression in thymocytes and percentages of DP and CD4SP or CD8SP thymocytes are shown. (B) Normal DP thymocyte apoptosis induced by OVA peptide in Runx1^F/F;Cd4-cre mice with the DO11.10 TCR transgene. Thymocytes from Runx1^F/F;Cd4-cre and littermate Runx1^F/F mice with the DO11.10 TCR transgene were incubated overnight in the presence of A20 antigen-presenting cells and different concentrations of OVA peptide. Cell viability was determined by Annexin V/propidium iodide staining, and viability relative to nontreated cells is shown. Data are shown as the mean and standard deviation from experiments performed in triplicate and represent data taken from four independent mice with similar results.