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Figure S4. CCL3 from CD8⁺ T cells is required for secondary protective immunity. PBS-injected or primary immunized (3 x 10³ WT bacteria) WT or CCL3^-/- mice (8-12 per group) were secondary challenged (3 x 10⁵). Purified CD8⁺ T cells were transferred into CCL3^-/- or WT mice. Recipient animals were further treated with anti-CCL3 or control serum and challenged. Data show the protective effect conferred by transferred CD8⁺ T cells in percentage (mean ± SD) in the spleen 2 d later and result from the pool of three experiments. 100% correspond to the protection conferred by the transfer of WT CD8⁺ T cells from secondary infected animals. p-values are indicated (n = 7).