Figure S1. Cytokine profile of human MSCs. 5 x 104 human MSCs, HUVECs, or KS cells were plated in 2 ml of appropriate growth media for 48 h. Media was harvested and assayed for the production of a panel of cytokines. As previously described, human MSCs synthesize substantial levels of the proangiogenic cytokines MCP-1 and VEGF-A (Kinnaird, T., E. Stabile, M.S. Burnett, C.W. Lee, S. Barr, S. Fuchs, and S.E. Epstein. 2004. Circ. Res. 94:678-685). On the other hand, MSCs produce very low levels of the pro-inflammatory cytokines IL-6 and IL-8 as compared with levels produced by KS cells. Values shown represent data from an individual experiment with all conditions performed in triplicate and are representative of two independent experiments.