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Figure S1. A prokaryotic Kv with essential sequence conservation of the voltage sensor. Clustal X alignment of the sequence of a prokaryotic Kv candidate from the genome of L. monocytogenes (KvLm) with 19 sequences of known Kv channels and 53 sequences from prokaryotic genomes postulated to encode for Kv channels. The conservation score shown below the alignment was calculated by Clustal X. Magenta shading indicates residues considered to be transmembrane α-helices as inferred from the crystal structure of KvAP (Jiang et al., 2003a). Conserved residues known to play a role in voltage sensing or K⁺ selectivity in Kv channels are shaded red (acidic), blue (basic), and green (uncharged). Residue numbers are for KvLm; “/” indicates sequence breaks. The abbreviated sequence names identify channels with the sequence accession numbers shown.