Molecular Determinant for Specific Ca/Ba Selectivity Profiles of Low and High Threshold Ca2+ Channels -- Data Supplement - JGP--Cens et al. -- The Journal of General Physiology

TABLE S1. Effects of Mutations at the DCS Locus on Cd²⁺ Block of Ba²⁺ Current and Current Reversal Potentials in 10 mM Ba²⁺ or Ca²⁺

Mutants channel	EC50 (Cd²⁺)	E_Rev (Ba²⁺)	E_Rev (Ca²⁺)
	µM	mV	mV
Ca_V2.3 WT [12]	2 ± 1	65 ± 2	73 ± 2
Ca_V2.3(DSED) [10]	3 ± 1	65 ± 1	74 ± 2
Ca_V2.3(TSQN) [11]	10 ± 1	65 ± 1	75 ± 1
Ca_V2.3(DEEE) [7]	2 ± 1	66 ± 1	74 ± 2
EC50 for the inhibition of Ba²⁺ currents by Cd²⁺ were obtained from dose–response curves realized in 10 mM Ba²⁺ bathing solution using increasing concentration of Cd²⁺, as in Fig. 4 C. Reversal potential was measured on current–voltage curves realized using voltage ramps from -80 mV to +80 mV (2.5 V/s) in 10 mM Ba²⁺ or Ca²⁺ bathing solutions.