TABLE S1. Amiloride Ki' of MEC-4/MEC-10 Heteromersa

MEC-4(713X)
MEC-10(673X)
Ki' @ -60 mV (µM)
Ki' @ 0 mV1 (µM)
δ
A
A
0.71 ± 0.02 (3)
1.9 ± 0.2 (3)
0.39 ± 0.05 (3)
A
C
0.38 ± 0.02 (6)a
1.06 ± 0.07 (6)
0.42 ± 0.02 (6)
A
D
0.65 ± 0.04 (12)
1.07 ± 0.07 (12)
0.19 ± 0.02 (12)
A
T
0.6 ± 0.2 (3)
2 ± 1 (3)
0.5 ± 0.1 (3)
A
V
0.67 ± 0.03 (3)
1.8 ± 0.2 (3)
0.42 ± 0.07 (3)
C
A
0.59 ± 0.02 (10)
1.9 ± 0.1 (10)
0.54 ± 0.05 (10)
C
C
0.36 ± 0.06 (6)a
1.0 ± 0.3 (6)
0.42 ± 0.05 (6)
D
A
2.17 ± 0.09 (11)
9.2 ± 0.7 (10)
0.65 ± 0.03 (10)
D
D
0.78 ± 0.04 (12)
3.5 ± 0.2 (11)
0.48 ± 0.06 (11)
T
A
2.7 ± 0.3 (20)
10.8 ± 0.7 (20)
0.69 ± 0.04 (20)
T
Tb
0.40 ± 0.03 (21)
1.5 ± 0.1 (18)
0.64 ± 0.04 (18)
V
A
0.42 ± 0.03 (10)
1.6 ± 0.1 (9)
0.65 ± 0.06 (9)
V
V
0.32 ± 0.02 (9)
1.5 ± 0.1 (9)
0.55 ± 0.02 (9)

aValues at -60 were measured directly, while Ki' at 0 mV was derived by fitting plots of Ki' vs. voltage with the Woodhull model. δ is the slope factor derived from the Woodhull model.

bData adapted from Goodman, M.B., G.G. Ernstrom, D.S. Chelur, R. O'Hagan, C.A. Yao, and M. Chalfie. 2002. Nature. 415:1039–1042.