McLaughlin et al. 10.1073/pnas.0710532105.
Fig. 6. Southern blot analysis of infected and sorted cells shows no difference in number of integrated genomes from single, double, or triple miRNAs. Ten micrograms of genomic DNA from Ba/F3 Fluc-Neo cells expressing p210 IRES-EYFP and infected with miRNA single 2 (lane 1), miRNA double 6/2 (lane 2), miRNA triple 6/2/1 (lane 3), and miRNA scrambled (lane 4) was digested with BamH1, run on a 1% agarose gel, blotted, and probed with GFP. Arrows indicate positions of BamH1 sites. A band of 1.4 kb (A) from the integrated genome of the lentiviral vector FC EmGFP W miRNA ABL hybridizes with the probe. Additionally, a 6-kb band (B) from MSCV-p210-IRES-EYFP hybridizes to the GFP probe because of cross-reactivity between GFP and YFP.
Fig. 7. Northern blot analysis of infected and sorted cells shows knockdown of p210 Bcr-Abl transcript. Twenty micrograms of total RNA from Ba/F3 Fluc-Neo cells expressing p210-IRES-EYFP and infected with miRNA single 2 (lane 1), miRNA double 6/2 (lane 2), miRNA triple 6/2/1 (lane 3), and miRNA scrambled (lane 4) was fractionated on a 1% agarose-formaldehyde gel, blotted and probe with a fragment of cABL cDNA. Blot was also probed with actin to show equivalent loading.