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Figure S1. ^{Ipl1 localizationissimilar to chromosomal passenger proteins.Chromosome spreads(SBY617) were stainedwith DAPI to recognize DNA (left) with or without(control) anti-Ipl1p antibodies, andwith anti-myc antibodiesto recognize the kinetochore proteinCse4-myc12.Inthe two bottom rows, spreadswere stainedwith anti-Tub1p antibodiesto recognize the spindle.The mergedimages(right) show thatendogenousIpl1p exhibitsthe same localizationpatternasIpl1-GFP andlocalizesto the kinetochoresinmetaphase andto the spindle andspindle midzone during anaphase.Bar, 10 &microm.}