Table S1. Antibody reagents.
| Antigen | Antibody name | Species and isotype | Obtained from |
Antibodies against HIV-1 proteins | |||
Matrix (p17) | 4C9 (ARP342) | Mouse IgG2a | R.B. Ferns and R.S. Teddera |
Capsid (p24) | 38:96K (EVA365) | Mouse IgG1 | B Wahrena |
Capsid (p24) | EF7 (EVA366) | Mouse IgG1 | B. Wahrena |
Capsid (p24) | Code D7320 | Sheep polyclonal | Aalto Bio Reagents, Ltd. |
Capsid (p24) | EH12-AP (ARP452) | Mouse IgG1 | J. Moorea |
Envelope gp120 | IAM 2G12 (EVA3064) | Human IgG1 | H. Katingera |
Envelope gp120 | IgG1 b12 (EVA3065) | Human IgG1 | D.P. Burton and P. Parrena |
Antibodies against cellular proteins | |||
HLA-DR | L243 | Mouse IgG2a | BD Biosciences |
CD63 | 1B5 | Mouse IgG2b | Prepared in houseb |
CD63 | FC5.01 | Mouse IgG2a | Zymed Laboratories |
LAMP-1 | Rabbit polyclonal | S. Carlsson, Umeå University, Umeå, Sweden | |
LAMP-2 | Rabbit polyclonal | S. Carlsson, Umeå University, Umeå, Sweden | |
CD68 | PG-M1 | Mouse IgG3 | Dako, Ltd. |
CD68 | KP-1 | Mouse IgG1 | R. da Silva, University of Oxford, Oxford, UK |
CD81 | M38 | Mouse IgG1 | F. Berditchevski, University of Birmingham, Birmingham, UK |
CD82 | M104 | Mouse IgG1 | F. Berditchevski, University of Birmingham, Birmingham, UK |
CD53 | MEM-53 | Mouse IgG1 | Abcam, Ltd. |
CD151 | 5C11 | Mouse IgG1 | F. Berditchevski, University of Birmingham, Birmingham, UK |
CD14 | UCHM1 | Mouse IgG2a | Serotec |
CD52 | CAMPATH-1G | Rat IgG2b | Serotec |
CD55 | MCA 914 | Mouse IgG1 | Serotec |
CD59 | MEM-43 | Mouse IgG2a | Abcam, Ltd. |
CD11a | MEM-25 | Mouse IgG1 | Abcam, Ltd. |
CD54 | MEM-111 | Mouse IgG2a | Abcam, Ltd. |
CD169 | 7D2 | Mouse IgG1 | P. Crocker, University of Dundee, Dundee, UK |
CD163 | GHI/61 | Mouse IgG1 | K. Pulford, University of Oxford, Oxford, UK |
Others | |||
| VSV-G protein | P5D4 | Mouse IgG1 | T. Kreis, University of Geneva, Geneva, Switzerland |
Secondary antibodies | |||
Mouse IgG | Rabbit polyclonal | Dako, Ltd. | |
Mouse IgG | Alexa® Fluor 488 | Goat | Molecular Probes, Inc. |
Mouse IgG | ß-Galactosidase | Goat | Southern Biotechnology Associates, Inc. |
Rat IgG H+L | Rhodamine | Goat | Perbio Science UK, Ltd. |
Rabbit IgG H+L | Rhodamine | Goat | Perbio Science UK, Ltd. |
| aObtained from the National Institute for Biological Standards and Control (NIBSC) Centralised Facility for AIDS Reagents; supported by the EU Programme European Vaccine Against AIDS (EVA) and the UK Medical Research Council. bFraile-Ramos, A., T.N. Kledal, A. Pelchen-Matthews, K. Bowers, T.W. Schwartz, and M. Marsh. 2001. The human cytomegalovirus US28 protein is located in endocytic vesicles and undergoes constitutive endocytosis and recycling. Mol. Biol. Cell. 12:1737-1749. | |||