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Figure S1. FAK knock down regulates MEK1 S298 phosphorylation. REF52 cells were treated with short interfering RNA (siRNA) oligomers specific to FAK for 72 h. Oligomers for luciferase served as a negative control. Untransfected cells or those transfected with siRNA were suspended for 60 min (S) and plated on 10 µg/ml FN for 10, 30, or 60 min, lysed, and blotted for MEK1 S298 phosphorylation (pS298MEK1), endogenous MEK1 (Transduction Laboratories, Inc), endogenous ERK2 (B3B9), or endogenous FAK (Transduction Laboratories, Inc.).