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Video 3 (1.0 MB)
A medial spot of Cdc15p-GFP arrives before ring formation and appears to nucleate Cdc15p ring assembly. Time-lapse images of live cdc15-GFP cells. Z-series optical sections (spacing of 0.5 µm) were collected at 25-s intervals. The cell shown in Fig. 4 A can be seen in the center of the frame. In this cell, a Cdc15p medial spot can be seen arriving with the subsequent formation of a ring.