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Figure S4. Deletion analysis of periplasmic ectodomain of Sln1. (A) The liquid cultures of strains transformed with centromeric plasmids encoding the wild-type SLN1 or the mutant SLN1 genes, with partly overlapping deletions (60 amino acids each) in the periplasmic domain (sln1 through sln19), were dropped onto plates containing galactose (GAL) or glucose (GLC) and tested for complementation of the sln1 strain (TM182). (B) Alignment of the putative N-linked glycosylation sites in an essential region of the periplasmic domain of Sln1 (aa 138-150) with sequences of related histidine kinases. Sequences were compared using the multiple sequence alignment tool Clustal W (Corpet, F. 1988. Nucleic Acids Res. 16:10881-10890).