Video 4 (2.7 MB)
Secretory granule motion observed by evanescent wave fluorescence microscopy. A PC-12 cell was cotransfected by NPY-GFP and the Rab binding domain of MyRIP (MyRIP-RBD) and imaged 3 d later by EW-FM. False color is used. Images were collected at 2 frames/s for 60 s, and the sequence is accelerated 5 times on the video (10 frames/s).