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Figure S3. The endogenous PrP^C did not colocalize with the transferrin receptor. CHO/30C3 cells were double immunolabeled with anti-PrP^C (R1) 5-nm gold and antitransferin antibodies (15-nm gold) on ultrathin cryosections. PrP^C (small gold) labeling was enriched in pericentriolar structures that did not colocalize with transferrin receptor-positive structures (large gold, arrows). n, nucleus.