[View Larger Version of this Image]

Figure S4. (A-C) Human GPI-anchored CD59 proteins in CHO cells under steady state do not reside in caveolae. Uniform distribution of anti-CD59 on ER, Golgi complex, TGN (A), and the plasma membrane (B and C), no enrichment in caveolae (C). Clathrin-coated pits at the plasma membrane (B, arrow) labeled for anti-CD59. Enrichment was seen at steady state in late endocytic multivesicular bodies (B, arrowheads). c, centriole; n, nucleus; m, mitochondria; p, plasma membrane.