Table 4.

Genes from clusters 1–5 with unassigned or unknown function found only in the testis (Unigene database)

Affymetrix probe id

Unigene id

Cluster

Predicted function /domains*

SP/TM

Confirmed by in situ hybridization

Confirmed by qRT-PCR

169602_f_at

Mm.117198

1

protease

   

167607_at

Mm.142735

1

    

169081_at

Mm.159029

1

    

167788_at

Mm.159154

1

MAGE

   

169574_at

Mm.159333

1

    

131914_at

Mm.188575

1

    

166295_f_at

Mm.212736

1

    

113853_at

Mm.22851

1

    

128554_at

Mm.248012

1

    

163896_at

Mm.249997

1

ER lumen protein retaining receptor

2 TM

  

162654_at

Mm.26189

1

    

133985_at

Mm.33633

1

    

165833_at

Mm.45462

1

    

165890_at

Mm.46124

1

    

165891_i_at

Mm.46128

1

    

161065_at

Mm.5349

1

protease

SP

  

167393_r_at

Mm.55950

1

 

TM

  

169326_f_at

Mm.63867

1

    

169687_at

Mm.69406

1

    

167791_at

Mm.78654

1

    

167928_at

Mm.84264

1

 

TM

  

167386_at

Mm.108732

2

    

166585_at

Mm.11439

2

    

168263_f_at

Mm.116959

2

citrate synthase

SP

  

165463_at

Mm.154333

2

    

166324_at

Mm.158195

2

    

167503_i_at

Mm.177848

2

    

165595_at

Mm.189648

2

protease

SP

  

108884_at

Mm.192221

2

    

168637_f_at

Mm.232593

2

MARVEL

4 TM

  

106254_at

Mm.23791

2

    

168050_at

Mm.250451

2

    

116993_at

Mm.251410

2

    

133351_at

Mm.25846

2

 

4 TM

  

165436_r_at

Mm.26987

2

poly A binding

   

166381_f_at

Mm.32219

2

 

7 TM

  

117078_at

Mm.33196

2

    

134003_at

Mm.33684

2

    

164130_at

Mm.40023

2

    

163005_s_at

Mm.45373

2

    

166239_f_at

Mm.45463

2

    

167012_at

Mm.45470

2

    

167057_i_at

Mm.45616

2

ubiquitin conjugating enzyme

   

163024_at

Mm.46106

2

Kazal

SP

  

163728_at

Mm.46140

2

    

165895_at

Mm.46142

2

    

165896_i_at

Mm.46144

2

    

171333_at

Mm.50085

2

    

168134_f_at

Mm.50108

2

    

166036_f_at

Mm.50853

2

    

168573_at

Mm.54267

2

    

170147_r_at

Mm.54292

2

 

TM

  

170426_f_at

Mm.63782

2

    

100965_at

Mm.660

2

    

167533_at

Mm.66809

2

 

TM

  

167538_at

Mm.66949

2

    

166156_f_at

Mm.67665

2

    

166172_f_at

Mm.71094

2

 

3 TM

  

166192_at

Mm.74465

2

    

166237_f_at

Mm.78558

2

    

166251_f_at

Mm.79179

2

transferase

   

168650_at

Mm.82793

2

    

167929_at

Mm.84275

2

    

167931_f_at

Mm.84314

2

    

101839_at

Mm.84934

2

protease

SP

  

165522_r_at

Mm.84977

2

    

166336_at

Mm.87044

2

    

163996_at

Mm.87382

2

    

135057_at

Mm.87734

2

    

168235_at

Mm.159080

3

    

168773_at

Mm.159449

3

    

167647_r_at

Mm.171293

3

    

129081_at

Mm.17612

3

    

138191_f_at

Mm.177680

3

    

108798_at

Mm.23366

3

    

114203_at

Mm.23521

3

    

165593_at

Mm.23534

3

Tektin

SP

  

164281_at

Mm.250814

3

    

133837_at

Mm.27514

3

    

166690_i_at

Mm.32241

3

    

114762_at

Mm.33646

3

    

163496_i_at

Mm.35644

3

   

also in ovary

136175_at

Mm.39873

3

    

139287_at

Mm.44817

3

    

107927_at

Mm.45587

3

    

167125_i_at

Mm.45821

3

 

4 TM

  

167128_r_at

Mm.45825

3

    

165893_f_at

Mm.46132

3

    

165888_f_at

Mm.46155

3

   

+

168783_f_at

Mm.48850

3

    

167645_f_at

Mm.48860

3

CD20/IgE Fc receptor

2 TM

  

114204_at

Mm.50600

3

 

SP/TM

  

167351_f_at

Mm.52646

3

    

163836_at

Mm.55669

3

sugar transporter

8 TM

  

165881_f_at

Mm.60992

3

    

167509_i_at

Mm.65550

3

    

116502_at

Mm.67234

3

 

2 TM

  

166151_f_at

Mm.67562

3

    

167654_i_at

Mm.72785

3

    

167662_r_at

Mm.73086

3

    

163094_at

Mm.78624

3

    

136447_at

Mm.83569

3

    

129903_at

Mm.84307

3

    

167961_r_at

Mm.85037

3

    

166359_at

Mm.87331

3

    

166366_r_at

Mm.87354

3

    

166367_at

Mm.87355

3

    

168262_at

Mm.87419

3

protease

TM

+

+

168254_at

Mm.109099

4

   

also in lung, ovary

165480_i_at

Mm.141451

4

calmodulin-binding

  

+

138799_at

Mm.158111

4

intermediate filament

  

+

168138_at

Mm.158149

4

   

+

170417_at

Mm.158159

4

    

169972_at

Mm.159092

4

   

+

167317_f_at

Mm.177847

4

   

+

166378_at

Mm.179421

4

    

115716_at

Mm.19977

4

protease

SP

  

115733_at

Mm.20221

4

histone-like

  

also in brain, heart

103632_at

Mm.20314

4

HMG group

   

168056_at

Mm.229259

4

    

163176_at

Mm.22956

4

   

+

131857_at

Mm.23382

4

    

168047_at

Mm.233840

4

 

TM

 

+

165592_r_at

Mm.23518

4

   

+

162601_i_at

Mm.23887

4

histone-like

  

+

107780_at

Mm.260945

4

    

114961_at

Mm.26988

4

lysozyme

SP

 

+

165439_at

Mm.27023

4

   

+

162777_at

Mm.29788

4

    

115963_at

Mm.30957

4

protease

SP

  

116183_at

Mm.31410

4

   

+

116186_at

Mm.31415

4

   

+

165661_i_at

Mm.32231

4

    

163401_at

Mm.32245

4

   

+

166730_at

Mm.33624

4

    

114760_at

Mm.33629

4

 

SP

 

+

166731_at

Mm.33647

4

    

114763_at

Mm.33683

4

    

114785_at

Mm.33811

4

    

163449_at

Mm.33864

4

    

105042_at

Mm.34841

4

   

+

165822_f_at

Mm.45302

4

 

SP

+

+

165824_at

Mm.45306

4

electron transporter

4 TM

+

+

163006_at

Mm.45377

4

sterol carrier

  

+

165831_at

Mm.45442

4

   

+

164171_at

Mm.45611

4

  

+

+

165857_at

Mm.45820

4

 

TM

  

165474_f_at

Mm.45824

4

   

+

165860_at

Mm.45831

4

   

+

165889_f_at

Mm.46121

4

  

+

+

163729_at

Mm.46146

4

histone-like

   

165900_f_at

Mm.46151

4

  

+

+

165486_i_at

Mm.46159

4

Ring finger

  

+

163032_at

Mm.46166

4

   

+

165487_f_at

Mm.46167

4

cystatin-like

SP

 

+

167439_at

Mm.46170

4

    

167318_i_at

Mm.50087

4

   

+

163819_at

Mm.50982

4

   

+

171461_f_at

Mm.52671

4

   

+

166061_f_at

Mm.55870

4

Phosphate acyltransferase, EF-hand, calcium binding

TM

 

+

166108_r_at

Mm.56430

4

HMG group

  

+

166073_at

Mm.56501

4

protease associated, Ring finger

SP

+

+

167423_f_at

Mm.59225

4

    

166097_at

Mm.60688

4

    

166104_f_at

Mm.61148

4

    

166147_f_at

Mm.67211

4

    

166191_f_at

Mm.72892

4

   

+

166195_f_at

Mm.73222

4

   

+

167775_r_at

Mm.78172

4

    

167780_i_at

Mm.78368

4

ARM repeat

  

+

163921_at

Mm.78390

4

   

+

166244_f_at

Mm.78873

4

    

166249_r_at

Mm.79053

4

    

167888_at

Mm.82810

4

    

167924_at

Mm.84066

4

   

+

167943_f_at

Mm.84522

4

  

+

 

110607_at

Mm.86429

4

    

166326_at

Mm.86808

4

   

+

168146_at

Mm.87067

4

    

166368_at

Mm.87357

4

electron transporter

   

166371_i_at

Mm.87369

4

ribonuclease inhibitor

   

166385_i_at

Mm.87418

4

    

168231_at

Mm.87431

4

    

164008_at

Mm.87446

4

   

also in lung

166397_at

Mm.87455

4

    

164787_at

Mm.157047

5

    

171469_r_at

Mm.158268

5

 

3 TM

  

169165_i_at

Mm.159335

5

    

168253_f_at

Mm.183467

5

    

168592_at

Mm.195787

5

protease

   

108744_at

Mm.23033

5

 

SP

  

161224_f_at

Mm.232542

5

    

165590_i_at

Mm.23510

5

    

165591_f_at

Mm.23516

5

   

+

163733_at

Mm.249241

5

    

134551_i_at

Mm.250837

5

    

166355_f_at

Mm.251374

5

    

115582_at

Mm.30493

5

    

162827_at

Mm.33194

5

    

136151_at

Mm.35312

5

    

171254_r_at

Mm.45304

5

 

TM

  

165834_r_at

Mm.45466

5

DNA binding

   

164176_at

Mm.45773

5

    

167124_at

Mm.45819

5

    

165883_r_at

Mm.46096

5

    

167848_at

Mm.50263

5

    

166026_r_at

Mm.50337

5

    

132023_at

Mm.50361

5

    

169155_f_at

Mm.50369

5

    

136448_at

Mm.50904

5

    

167346_f_at

Mm.52575

5

    

167349_i_at

Mm.52613

5

    

99400_at

Mm.56997

5

 

SP

  

166083_i_at

Mm.57614

5

    

167531_i_at

Mm.60359

5

    

167537_at

Mm.66946

5

    

163877_at

Mm.67455

5

    

166154_f_at

Mm.67595

5

    

163081_at

Mm.67639

5

    

165901_f_at

Mm.71110

5

    

167602_i_at

Mm.71153

5

    

167605_i_at

Mm.71176

5

    

170163_at

Mm.71904

5

    

130175_at

Mm.71906

5

    

166193_at

Mm.72970

5

  

+

+

167659_at

Mm.73124

5

    

167699_at

Mm.75060

5

    

170614_at

Mm.78473

5

    

133077_at

Mm.81032

5

   

+

167939_s_at

Mm.84467

5

    

167954_at

Mm.84810

5

    

163973_at

Mm.86986

5

    

166390_at

Mm.87434

5

    

A signal peptide (SP) or transmembrane segment (TM) was predicted by using signalp, Ver. 2.0 (www.cbs.dtu.dk/services/SignalP-2.0); potential functional domains were assigned on the basis of gene ontology information (netaffx), smart (http://smart.embl-heidelberg.de), and scanprosite (http://us.expasy.org/tools/scanprosite). HMG, high-mobility group; Kazal, serine protease inhibitor; ARM, Armadillo/beta-catenin-like repeats; MAGE, Similar to Melanoma Antigen family; MARVEL, domain involved in membrane apposition events.