Table 1. Cell cycle distribution of LT-pretreated RAW 264.7 macrophages

Conditions*

%G0/G1

%S

%G2/M

Control

73.21 ± 9.7

20.54 ± 9.16

6.25 ± 0.54

PA/LF

88.30 ± 0.41

7.93 ± 0.89

3.71 ± 0.56

*, RAW 264.7 were left untreated (control) or incubated 24 h with doses of LT- (PA 1 m g/ml and LF 5 ng/ml) inducing TIR. Cells were then stained with propidium iodide and analyzed by flow cytometry. Data represent the average of two separate experiments (± SE).