Fig. 9.
Levels of total proteins in TIR and non-TIR RAW 264.7. RAW 264.7 cells were pretreated as previously described and rechallenge with a high dose of LT (1 m g/ml PA and LF). Samples were run on 10% SDS/PAGE and Coomassie-stained. Lanes 1 and 9, M: SeeBlue Plus2 prestained standard (Invitrogen); lane 2, mock-treated control; lanes 3 and 4, pretreated low dose; lanes 5?8, 10, and 11, TIR macrophages treated with high-dose LT (1 m g/ml PA and LF); lane 12, mock-treated control; lanes 13-15, non-TIR macrophages treated with high dose of LT; lane 16, mock-treated control. Time corresponds to LT high-dose treatment unless indicated by *, where time is relative to initial treatment.