Table 3. Oligo-DNA primers used for this study
Name | Sequence* | Construct |
3HA1f | TACCCATACGATGTTCCTGACTATGCG | † |
3HA2r | AGCGTAATCTGGAACGTCATATGGATA | † |
TpAtx-2r | ACGCGTCGACtcaAGCCTTACTAAGATCAGGAGGGTTTCTC | All pHpl |
TpAtx-3f | AATGAATTCatgTTGGATTCTCTAGTGTCGAAACTGCCTTC | All pHpl |
HTpAtx-1f | AATGAAATTCatgTACCCATACGATGTTCCTGACTATGCG | pHpl-1 |
HTpAtx-2f | TATCCATATGACGTTCCAGATTACGCT ATGTTGGATTCTCTAGTGTCGAAACTGCCTTC | pHpl-1 |
HTpAtx-3r | CGCATAGTCAGGAACATCGTATGGGTA AGCCTTACTAAGATCAGGAGGGTTTCTC | pHpl-13 |
HTpAtx-4r | ACGCGTCGACtcaAGCGTAATCTGGAACGTCATATGGATA | pHpl-13 |
HTpAtx-5r | CGCATAGTCAGGAACATCGTATGGGTA CTCTTCCAAAAGATGACCAAGAACAATAC | pHpl-2 |
HTpAtx-6f | TATCCATATGACGTTCCAGATTACGCT AATAGATGGATGAACGAATCCATCACCGCCTTG | pHpl-2 |
HTpAtx-7r | CGCATAGTCAGGAACATCGTATGGGTA TTTTCCTTTACTAATCAACAAAATGGTAACAC | pHpl-3 |
HTpAtx-8f | TATCCATATGACGTTCCAGATTACGCT AGCTCGCATCTTCTCGTCTTTAGTGAAG | pHpl-3 |
HTpAtx-9r | CGCATAGTCAGGAACATCGTATGGGTA TTTTACTTGAAACCCTGCATTGAATATAATGG | pHpl-4 |
HTpAtx-10f | TATCCATATGACGTTCCAGATTACGCT AAGAAGCAGTTTTTCCGCAATTTCGTGAC | pHpl-4 |
HTpAtx-11r | CGCATAGTCAGGAACATCGTATGGGTA AATGTCCAACTTCTTAAAGAACTGTGTTAC | pHpl-5 |
HTpAtx-12f | TATCCATATGACGTTCCAGATTACGCT GGAACCTTTGACTTGGGTGATTATCTTG | pHpl-5 |
HTpAtx-13r | CGCATAGTCAGGAACATCGTATGGGTA CTCGTCTTGATTCAGAACCTGCAGTGTAC | pHpl-6 |
HTpAtx-14f | TATCCATATGACGTTCCAGATTACGCT ACACCTTTGCTTTACAGTCTTGTATTCGG | pHpl-6 |
HTpAtx-15r | CGCATAGTCAGGAACATCGTATGGGTA GTGAGTGAGATCAAAGCTCTGAATCGCG | pHpl-7 |
HTpAtx-16f | TATCCATATGACGTTCCAGATTACGCT CTAAACCACGAAGCTGCTTTTCATCTTC | pHpl-7 |
HTpAtx-17r | CGCATAGTCAGGAACATCGTATGGGTA TGAGTGCCTTCCAAAGTATAGCTTCTTG | pHpl-8 |
HTpAtx-18f | TATCCATATGACGTTCCAGATTACGCT ACTGACCGAGAGGTTGCCCTTATGATGC | pHpl-8 |
HTpAtx-19r | CGCATAGTCAGGAACATCGTATGGGTA TACATTGTGCCATGTGTAATGGGACATC | pHpl-9 |
HTpAtx-20f | TATCCATATGACGTTCCAGATTACGCT ACGGAGAGCTCAAGAATAACAACAAAGC | pHpl-9 |
HTpAtx-21r | CGCATAGTCAGGAACATCGTATGGGTA CCACTTGTCAATGTCCAAGGCATCCATTCC | pHpl-10 |
HTpAtx-22f | TATCCATATGACGTTCCAGATTACGCT AGATCCGTGAGTGACACACCGGGAACATCG | pHpl-10 |
HTpAtx-23r | CGCATAGTCAGGAACATCGTATGGGTA GATTTTCTCGCTTTGATTCTTCTTGGCATAAG | pHpl-11 |
HTpAtx-24f | TATCCATATGACGTTCCAGATTACGCT AACTTTAACATGGCAGGTTGTGATTTGGTG | pHpl-11 |
HTpAtx-25r | CGCATAGTCAGGAACATCGTATGGGTA AAACTTGTTGTATGCAAGAGCCATAGATAC | pHpl-12 |
HTpAtx-26f | TATCCATATGACGTTCCAGATTACGCT ACAAGGGCCGGGCACACAGATGTACGCG | pHpl-12 |
HTpAtx-27r | CGCATAGTCAGGAACATCGTATGGGTA GCTTATGAGTGGTTTGGTCAGCATACC | pHpl-13.2 |
HTpAtx-28f | TATCCATATGACGTTCCAGATTACGCT TACCTATTACCGCACCAGAACGCCACC | pHpl-13.2 |
Bam-X1f | ACGCGGATCCatgTTGGATTCTCTAGTGTC | CDL, X1‡ |
Eco-X1r | ACGGAATTCtcaAGCCTTACTAAGATCAG | NDL, X1‡ |
X1cd-R | AGCTGAATTCctaAGATAACATGCTCGTGGTG | CDL‡ |
X1ND-f | ACGTGGATCCatgGCTTCTGTGGTTGCGTTGAATC | NDL‡ |
*Restriction enzyme recognition sites are underlined, hemagglutinin (HA) sequence is shown as bold, and transcription initiation and termination codon are shown as lower case, respectively.
†
3HA1f and -2r were used only for amplifying the 3xHA fragment.‡
X1: AtNHX1 full length ORF; CDL, C terminus deletion; NDL, N terminus deletion.