Video 3 (604K)
A wild-type mitotic cell expressing GFP-tubulin. The mother cell is to the upper left and the bud is to the lower right. The movie starts with a bright-field image to show the cell contours and then switches to fluorescence to visualize GFP-tubulin. The bright bar-like spindle is in the mother cell, and astral microtubules are seen extending into the bud. The astral microtubules associate with the bud cortex "plaster" and then slide towards the bud tip. The sliding microtubules pull on the mitotic spindle, which rotates the spindle into the focal plane and moves the spindle into the neck (occurred in six of eight cells viewed). The movie is playing at 7.5 times real time (acquired at two frames per second [fps] and is playing back at 15 fps). Field is 9.3 µm wide by 11.2 µm high.