Structure and function of a membrane-bound murine MHC class I molecule -- Celia et al. 96 (10): 5634 Data Supplement - Supplemental Data -- Proceedings of the National Academy of Sciences

Supplementary material for Celia et al. (1999) Proc. Natl. Acad. Sci. USA 96(10), 5634-5639.

Three-Dimensional Reconstruction of H-2K^b
Fig. 5.
Amplitudes and phases along six different lattice rods used for the three-dimensional reconstruction of H-2K^b. The dots represent the amplitudes and phase values obtained from individuals images. The smooth curves were generated with the program LATLINE and are shown as solid lines. Fluorescence Recovery After Photobleaching

Experiments were performed on lipid monolayers supported on alkylated surfaces. The size of the photobleached box and the elapsed times after bleaching are indicated.

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Figs. 6, 7, and 8. Supported monolayer made of DOPC (dioleoyl phosphatidylcholine), DOGS-NTA.Ni [1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl)imidodiacetic acid)succinyl] (nickel salt)] nickel-chelating lipid, and NBD-PE [N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine] fluorescent lipid in a 8.5:1:0.5 wt/wt/wt ratio.

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Figs. 9 and 10. Same as Figs. 5, 6, and 7, but His-tagged H-2K^b was injected onto the surface at a final protein concentration of »0.4 mg/ml.

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Fig. 11. Same as Figs. 8 and 9, but performed on an area that was dried out, that is, where there was no buffer underneath the supported lipid monolayer. The lateral diffusion is abolished, and there is virtually no recovery of fluorescence. Also, the edges of the bleached box remain sharp, in contrast to the one observed for the other conditions.