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Video 5 (7.1 MB)
Serum add-back to PH-GFP-expressing cells.^{Additionof serum inducesmembrane ruffling, leading to the formationof PIP₂-positive macropinosomesthatexhibita dramatic lossof PIP₂ asthey migrate into the cell.Some macropinsomesremainat40 min.These all disappearedoncontinuedviewing (see Fig. 7 E).}