Supplementary material for Ball et al. (1999) Proc. Natl. Acad. Sci. USA 96(12), 6637-6642.

 

Table 2.EPR data and exposure factors for 13 spin-labeled C2cPLA2 mutants.

Site

DOPM

Crox

O2

e

P

2 (gauss2)*

D

P2 (gauss2)†

F

A34

+

-

-

   1.16

0.051 ± 0.002

 

 

 

+

-

+

   1.00

0.183 ± 0.005

 

 

 

+

+

-

   1.24

0.062 ± 0.002

0.011 ± 0.003

 

 

-

-

-

   1.28

0.067 ± 0.001

 

 

 

-

-

+

   1.14

0.174 ± 0.001

 

 

 

-

+

-

   1.04

0.362 ± 0.005

0.295 ± 0.005

0.04 ± 0.01

L39

+

-

-

   1.06

0.051 ± 0.001

 

 

 

+

-

+

   1.00

0.24 ± 0.01

 

 

 

+

+

-

   1.06‡

0.051 ± 0.006

0.000 ± 0.006

 

 

-

-

-

   1.26

0.066 ± 0.001

 

 

 

-

-

+

   1.16

0.163 ± 0.001

 

 

 

-

+

-

   1.09

0.378 ± 0.006

0.312 ± 0.006

0.00 ± 0.02

D55

+

-

-

   1.03

0.046 ± 0.002

 

 

 

+

+

-

   0.901

0.076 ± 0.008

0.030 ± 0.008

 

 

-

-

-

   1.19

0.068 ± 0.004

 

 

 

-

+

-

   1.04

0.530 ± 0.003

0.462 ± 0.005

0.155 ± 0.005

R61

+

-

-

   1.14

0.067 ± 0.001

 

 

 

+

+

-

   1.07

0.109 ± 0.001

0.042 ± 0.001

 

 

-

-

-

   1.17

0.0718 ± 0.0003

 

 

 

-

+

-

   1.10

0.341 ± 0.005

0.270 ± 0.005

0.155 ± 0.005

V70

+

-

-

   1.09

0.053 ± 0.002

 

 

 

+

-

+

   1.14

0.173 ± 0.003

 

 

 

+

+

-

   1.09

0.200 ± 0.004

0.147 ± 0.004

 

 

-

-

-

   1.14

0.059 ± 0.001

 

 

 

-

-

+

   1.12

0.179 ± 0.003

 

 

 

-

+

-

   1.05

0.334 ± 0.006

0.275 ± 0.007

0.53 ± 0.02

A78

+

-

-

   1.14

0.610 ± 0.009

 

 

 

+

+

-

   1.06

0.098 ± 0.001

0.037 ± 0.001

 

 

-

-

-

   1.20

0.075 ± 0.001

 

 

 

-

+

-

   1.03

0.274 ± 0.003

0.199 ± 0.003

0.186 ± 0.006

E88

+

-

-

   1.03

0.054 ± 0.003

 

 

 

+

+

-

   1.04

0.119 ± 0.005

0.065 ± 0.006

 

 

-

-

-

   1.08

0.050 ± 0.002

 

 

 

-

+

-

   1.04

0.331 ± 0.008

0.281 ± 0.008

0.23 ± 0.02

V97

+

-

-

   1.02

0.0359 ± 0.0009

 

 

 

+

-

+

   1.00‡

0.27 ± 0.01

 

 

 

+

+

-

   0.974

0.042 ± 0.002

0.006 ± 0.002

 

 

-

-

-

   1.15

0.051 ± 0.002

 

 

 

-

-

+

   0.972

0.136 ± 0.005

 

 

 

-

+

-

   1.02

0.350 ± 0.007

0.299 ± 0.007

0.02 ± 0.02

S110

+

-

-

   1.06

0.061 ± 0.003

 

 

 

+

+

-

   0.997

0.253 ± 0.004

0.192 ± 0.005

 

 

-

-

-

   1.03

0.059 ± 0.003

 

 

 

-

+

-

   0.992

0.375 ± 0.007

0.316 ± 0.008

0.61 ± 0.02

K113

+

-

-

   0.913

0.046 ± 0.002

 

 

 

+

+

-

   0.872

0.109 ± 0.007

0.063 ± 0.007

 

 

-

-

-

   0.890

0.055 ± 0.002

 

 

 

-

+

-

   0.921

0.286 ± 0.006

0.231 ± 0.006

0.27 ± 0.03

K117

+

-

-

   1.16

0.0681 ± 0.0004

 

 

 

+

+

-

   1.16

0.23 ± 0.01

0.16 ± 0.01

 

 

-

-

-

   1.15

0.069 ± 0.001

 

 

 

-

+

-

   1.14

0.281 ± 0.003

0.212 ± 0.003

0.75 ± 0.05

Q126

+

-

-

   1.12

0.052 ± 0.007

 

 

 

+

+

-

   1.08

0.095 ± 0.002

0.043 ± 0.007

 

 

-

-

-

   1.24

0.0638 ± 0.0005

 

 

 

-

+

-

   1.06

0.413 ± 0.002

0.349 ± 0.002

0.12 ± 0.02

C139

+

-

-

   1.12

0.052 ± 0.002

 

 

 

+

+

-

   1.06

0.167 ± 0.004

0.115 ± 0.004

 

 

-

-

-

   1.12

0.057 ± 0.004

0.115 ± 0.004

 

 

-

+

-

   1.00

0.376 ± 0.005

0.319 ± 0.006

0.36 ± 0.01

 

*e and P2 are from equation 1 of Lin et al. [Lin, Y., Nielsen, R., Murray, D., Mailer, C., Hubbell, W. L., Robinson, B. H. & Gelb, M. H. (1998) Science 279, 1925-1929].

†

DP2 as calculated by equation 5 of Lin et al.

‡

This value was fixed during the fit to be consistent with other values.