A2A receptor signaling promotes peripheral tolerance by inducing T-cell anergy and the generation of adaptive regulatory T cells -- Zarek et al. 111 (1): 251 Data Supplement - <font size="3">Supplemental materials for: Zarek et al</font> -- Blood

Blood, Vol. 111, Issue 1, 251-259, January 1, 2008

A_2A receptor signaling promotes peripheral tolerance by inducing T-cell anergy and the generation of adaptive regulatory T cells
Blood Zarek et al. 111: 251

Supplemental materials for: Zarek et al

Files in this Data Supplement:

Figure S1. A_2AR expression is upregulated under conditions that promote anergy, in vitro (PDF, 30 KB) -
(A) IL-2 production of A.E7s rechallenged with anti-CD3 + anti-CD28 that had either been previously mock stimulated, stimulated with anti-CD3, or anti-CD3+CSA. (B) In vitro A_2AR mRNA expression as determined by microarray analysis for A.E7 cells that were mock-stimulated, stimulated with anti-CD3, or anti-CD3+CSA. The Y axis is expression as determined by the Affymetrix algorithm. In general values
Figure S2. Equivalent clonotypic T cells in the CGS and vehicle treated mice (PDF, 62 KB) -
6.5⁺ T cells were adoptively transferred into C3HA mice and treated with vehicle or CGS as described in Figure 5. Splenocytes were harvested on Day 2 and Day 3 post transfer and evaluated for percent clonotypic 6.5⁺ T cells. Data are representative of 10 mice, each condition.