Lack of iNKT cells in patients with combined immune deficiency due to hypomorphic RAG mutations -- Matangkasombut et al. 111 (1): 271 Data Supplement - <font size="3">Supplemental materials for: Matangkasombut et al</font> -- Blood

Blood, Vol. 111, Issue 1, 271-274, January 1, 2008

Lack of iNKT cells in patients with combined immune deficiency due to hypomorphic RAG mutations
Blood Matangkasombut et al. 111: 271

Supplemental materials for: Matangkasombut et al

Files in this Data Supplement:

Figure S1. Flow cytometric analysis of peripheral blood mononuclear cells (PBMC) from 6 healthy controls (JPG, 58.4 KB) -
Leukocytes were enumerated by gating on CD45⁺CD3⁺ cells. iNKT cells were identified using PBS57-loaded CD1d tetramers (right panel) using cells stained with unloaded CD1d tetramers as control (left panel). In the 6 controls, iNKT cells represent between 0.1 to 0.7 % of CD3⁺ cells (from 547 to 6265 iNKT cells per ml of blood).
Figure S2. Flow cytometric analysis of peripheral blood mononuclear cells (PBMC) from patient 3 and 5 and a healthy control using three different methods to identify iNKT cells (JPG, 93.5 KB) -
Leukocytes were enumerated by gating on CD45⁺CD3⁺ cells. iNKT cells were identified using a) PBS57-loaded CD1d tetramers (top left) using cells stained with unloaded CD1d tetramers as control (bottom left) b) anti-invariant NKT cells antibody (clone 6B11) (top middle) and isotype antibody control (bottom middle) and c) anti V24/V11 antibody (top right) and isotype antibody control (bottom right).