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Figure S4. 2× FYVE as a specific marker for engulfed cell corpses. (A) Time-lapse images indicating the process of engulfment and the presence of PI(3)P on the surface of nascent phagosomes in a wild-type embryo expressing both P_ced-1 ced-1::gfp and P_ced-12× FYVE::mrfp1. The anterior is shown on top and the ventral side faces outward. Insets show 3× views of the boxed regions. (B) Table summarizing the time interval between the completion of engulfment (CED-1::GFP circle formation) and the recruitment of 2× FYVE::mRFP1 to phagosomes in wild-type embryos. Data are presented as mean ± SD (n = 3). (C) DIC and corresponding fluorescent images of 2× FYVE::GFP in twofold stage embryos of different genotypes. The anterior is shown on top and the ventral side is shown on the left. Arrows indicate cell corpses. (D) The total numbers of DIC(+) cell corpses and 2× FYVE::GFP circles in twofold stage embryos of different genotypes. Results are presented as mean ± SD (n = 15). Bars, 5 µm.