[View Larger Version of this Image]

Figure S2. Radial distribution of chromosome territories after exit from the cell cycle in 2D cultures. (A) Whole chromosomes (red) were detected in PFA-fixed proliferating and quiescent 2D cultures. Nuclei were counterstained with DAPI (blue). Projected stacks of nuclei are shown. Bars, 5 μm. (B) Cumulative frequency graphs to quantify the radial distribution (RRD) for chromosomes (HSA) 8, 11, 14, or 17 in proliferating (Day 0) and quiescent 2D cultures of MCF-10A.B2 cells. Results are pairwise comparisons of cumulative radial distributions using a 1D KS test. n = 100 per chromosome per growth condition.