Time-resolved analysis and visualization of
dynamic processes in living cells

Wolfgang.Tvarusko{at}iwr.uni-heidelberg.demistelit{at}mail.nih.govRuediger.Rudolf{at}urz.uni-heidelberg.deKaether{at}embl-heidelberg.despector{at}cshl.orghhgerdes{at}sun0.urz.uni-heidelberg.deRoland.Eils{at}iwr.uni-heidelberg.de



Study of Gene Expression Events


As splicing of most pre-mRNAs occurs co-transcriptionally, peripheral movements were strictly dependent on ongoing RNA polymerase II transcription. If inhibitors of RNA polymerase II were added, neither peripheral extensions nor (dis)associating particles were observed.

JPEG Image The surrounding borderline of the detected speckle is coloured in green. The RNA polymerase II inhibitor caused a marked rounding up of the speckles and no peripheral movements were observed.

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JPEG Image Reconstructed 3D spatio-temporal structure of the speckle based on the extracted borderline of the 20 images (time lapse: 1 min). The highlighted rings along the reconstructed shapes correspond to outlines of the speckle at discrete time steps.

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JPEG Image 3D time-space reconstruction from a speckle corrected for the speckle movement.

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[ motility of secretory vesicles ]

[ simulation of vesicle dynamics and tracking ]

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