Rescue of Pituitary Function in a Mouse Model of Isolated Growth Hormone Deficiency Type II by RNA Interference
Endocrinology Shariat et al. 149: 580 Supplemental Data
Supplemental Data
Files in this Data Supplement:
- Supplemental Table 1 - Two sets (a, b) of pronuclear injections were performed to generate shRNA-17.5 transgenic founders. Mice in bold have been shown to rescue a mouse model of IGHD II. shRNA mice 25a and 20b were selected for further analysis and were subsequently referred to as sh-25 and sh-20 respectively.
- Supplemental Table 2 - Summary of 95% confidence intervals, comparison of both pairs by Tukey-Kramer HSD and Shapiro-Wilk’s goodness of fit test for normal distribution of the values at week 16 in Supplemental Figure 1. For Tukey-Kramer HSD test, groups with different letters are significantly different (α=0.001).
- Supplemental Table 3 - Summary of 95% confidence intervals, comparison of all pairs by Tukey-Kramer HSD and Shapiro-Wilk’s goodness of fit test for normality for values at week 16 in Figure 2b. For Tukey-Kramer HSD test, groups with different letters are significantly different (α=0.001).
- Supplemental Table 4 and Microscopy equipment and settings - Summary of 95% confidence intervals, comparison of all pairs by Tukey-Kramer HSD and Shapiro-Wilk’s goodness of fit test for normal distribution for values in Figure 2d. Values for weeks 8 and 16 are listed in the upper and lower panels, respectively. For Tukey-Kramer HSD test, groups with different letters are significantly different (α=0.001).
- Supplemental Figure 1 - Growth Curves of shRNA Mice. Weights of transgenic mice (sh-20 line (n=4), yellow; sh-25 line (n=6), green) and wild type littermates (n=5 for each graph) (blue) were determined between 4-16 weeks of age. Error bars are ±s.e.m. From ANOVA, p>0.7 for both data sets. Full statistical analyses are in Supplemental Table 2.
- Supplemental Figure 2 - Expression of shRNA17.5 does not elicit an interferon response. RNA was isolated from pituitaries of the indicated genotypes and RT-PCR was performed using primers against the interferon induced gene, 2’, 5’-oligoadenylate synthetase (OAS 1) (left) and β-actin as a control (right).
- Supplemental Figure 3 - Western blot analysis of GH isoforms from mouse pituitary lysates. A shorter exposure (2 seconds) of the same gel shown in Fig. 5 (15 seconds).