Y3+ Block Demonstrates an Intracellular Activation Gate for the {alpha}1G T-type Ca2+ Channel -- Data Supplement - JGP--Obejero-Paz et al. Table S1 -- The Journal of General Physiology

TABLE S1. Time Course of Closed-state block

Divalents	Y³⁺		₁	₂	A₁ / (A₁ + A₂)	N
mM	nM	ms	ms	ms
2 Ca²⁺, 1 Mg²⁺	100	604 ± 69	107 ± 30	774 ± 39	0.16 ± 0.05	9
2 Ca²⁺, 1 Mg²⁺	300	93 ± 20	33 ± 8	323 ± 27	0.47 ± 0.04	4
2 Ca²⁺, 1 Mg²⁺	1000	22 ± 2	12.1 ± 0.9	206 ± 33	0.71 ± 0.03	7
2 Ca²⁺, 1 Mg²⁺	3000	9.1 ± 0.8	4.7 ± 0.5	129 ± 35	0.70 ± 0.02	7
0.5 Ca²⁺	1000	13.1 ± 1.7	5.4 ± 0.3	69 ± 6	0.65 ± 0.05	10
2 Ca²⁺	1000	21 ± 3	9.0 ± 0.9	159 ± 21	0.64 ± 0.04	11
4 Ca²⁺	1000	53 ± 6	15.1 ± 1.6	256 ± 29	0.49 ± 0.03	7
0.5 Ba²⁺	1000	6.0 ± 1.4	2.7 ± 0.8	33 ± 7	0.64 ± 0.03	9
2 Ba²⁺	1000	7.1 ± 0.9	4.0 ± 0.4	83 ± 8	0.73 ± 0.01	13
4 Ba²⁺	1000	11.2 ± 0.8	5.6 ± 0.4	96 ± 16	0.67 ± 0.02	10
The concentrations of di- and trivalent cations in the extracellular solutions are given in the leftmost two columns. Data are from the protocol of Fig. 5. The time course of closed-state block at -100 mV (0 to 1024 ms) was fitted to a single exponential () plus an offset, or to the sum of two exponentials plus an offset, where A₁ and A₂ are the amplitudes of the fast (₁) and slow (₂) components, respectively.