[View Larger Version of this Image]

Figure S5. Isolation of box C/D core protein complex from different mutant strains. (A) Western blot analysis demonstrating that the levels of Rvb1 and Rvb2 proteins are reduced in rvb1-DAmP and rvb2-DAmP strains, respectively. Western blot against Hsp90 is shown as a loading control. All strains used in this figure have the 3xFLAG tag added at the 3′ end of chromosomal NOP56 gene. (B) Nop56–3FLAG protein complexes were immunoprecipitated from WT, tah1Δ, rvb1-DAmP, and rvb2-DAmP strains. Proteins were separated on an SDS-PAGE gel and were visualized by silver staining (top) or immunoblotted with αHsp90 and αFLAG antibodies (middle and bottom, respectively).