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Figure S1. Cellular localization of EVERs and ZnT-1. HaCaT cells transiently transfected with FLAG-ZnT-1 (A–C), FLAG-EVER1 (D–F), and FLAG-EVER2 (G–I) were incubated with a mixture of anti-FLAG mouse monoclonal antibodies and anti-calnexin rabbit antibodies. After washings, cells were stained with CY3-conjugated anti–mouse antibodies (red) and FITC-conjugated anti–rabbit antibodies (green). Merge image (yellow) is shown in C, F, and I. HaCaT cells constitutively expressing pCINeo (J), FLAG-EVER1 (K), FLAG-EVER2 (L), or FLAG-ZnT-1 (M) and HaCaT cells transiently transfected with ZnT-1 (N) or FLAG-ZnT-1 (O) were successively incubated with anti-FLAG mouse monoclonal antibodies and CY3-conjugated anti–mouse antibodies (red). Living HaCaT cells transiently transfected with GFP-ZnT-1 were visualized with a FITC filter before (P) adding zinc or after 5 (Q) and 20 min (R) incubation in 140 μM zinc. HeLa (S), CaSki (T), and SKV (U) keratinocytes were transfected with FLAG-ZnT-1 and successively incubated with anti-FLAG mouse monoclonal antibodies and CY3-conjugated anti–mouse antibodies (red). Bars, 10 μm.