Mosaic Complementation Demonstrates a Regulatory Role for Myosin VIIa in Actin Dynamics of Stereocilia
Mol. Cell. Biol. Prosser et al. 28: 1702 Supplemental material
Files in this Data Supplement:
- Supplemental file 1 - Appendix S1 text (Alternative genotyping for BAC transgenes by PCR)
Zipped MS Word document, 4 KB. - Supplemental file 2 - Appendix S1 figures (Fig. 1 [Schematic map of primer locations relative to the Hprt locus and integrated BAC] and 2 [Representative PCR genotyping])
Zipped PDF document, 5.2 MB. - Supplemental file 3 - Appendix S2 (Summary breakdown of mice used)
Zipped MS Word document, 8 KB. - Supplemental file 4 - Appendix S3 (Vector map of pCEI1)
Zipped PDF document, 729 KB. - Supplemental file 5 - Appendix S4 (Effect of BAC quantity of RMCE efficiency)
Zipped MS Word document, 5 KB. - Supplemental file 6 - Appendix S5 [Analysis for presence and integrity of BAC integration at the hprttm(rmce1)Brd locus for human BAC RPCI11-189K21]
Zipped PDF document, 6.2 MB. - Supplemental file 7 - Appendix S6 (Primer sequences for genomic PCR of integrated BAC inserts for zebrafish BAC RPCI 71-30I22 and human BAC RPCI 11-189K21)
Zipped MS Word document, 8 KB. - Supplemental file 8 - Appendix S7 (Myo7a-lacZ transcriptional reporter expressed from the X chromosome localizes to hair cells and shows cellular mosaicism in hemizygous transgenic females)
Zipped PDF document, 11.8 MB. - Supplemental file 9 - Appendix S8 (Integration of Myo7a BACs and germ line transmission)
Zipped MS Word document, 5 KB. - Supplemental file 10 - Appendix S9 (Summary of verification of complementation of Myo7a4626SB mutant mice by Myo7a containing BAC transgenes)
Zipped MS Word document, 6 KB.