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Figure S1. HSACre-ILK muscle displays centralized nuclei in different type of muscles. (A) Hematoxylin and eosin–stained paraffin sections of soleus, tibialis anterior (TA), and extensor digitorum longus (EDL) of 5-mo-old control and HSACre-ILK mice. Note the irregular diameter and centrally located nuclei (arrowheads) in myofibers of HSACre-ILK mice. Bar, 30 µm. (B) The number of myofibers with central nuclei increased from 7.2 ± 2.1% in 4-wk-old HSACre-ILK mice, to 11.6 ± 2.9% in 3-mo-old HSACre-ILK mice, to 22.2 ± 5.1% in 12-mo-old HSACre-ILK mice, whereas the numbers in control mice were ∼1.0 ± 0.3%, 1.1 ± 0.7, and 1.2 ± 0.6, respectively, at the ages analyzed. Data are expressed as mean ± SD (P < 0.01).