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Figure S2. Measurement of myofiber density and size. (A) The density of myofibers was defined by the number of myofibers per area (10,000 pixel²). GC, tibialis anterior (TA), and soleus muscles from 3- and 5-mo-old control and HSACre-ILK mice were measured. The soleus myofiber density was 150.4 ± 11.6 and 210.6 ± 29.8 in 3-mo-old control and HSACre-ILK (P < 0.001) mice, respectively, and 17.5 ± 1.8 and 27.5 ± 3.9 in 5-mo-old control and HSACre-ILK (P < 0.001) mice, respectively. Data are expressed as mean ± SD. (B) Myofiber size of GC, soleus, and TA muscles of 3- and 5-mo-old control and HSACre-ILK mice was measured by pixel² per myofiber and shown by dot-plots. The HSACre-ILK muscles show an altered distribution of fiber size with a prevalence of fibers with a smaller diameter.