Published February 25, 2008 // JCB vol. 180 no. 4 771-785
The Rockefeller University Press, doi: 10.1083/jcb.200709102

SAS-4 is recruited to a dynamic structure in newly forming centrioles that is stabilized by the γ-tubulin–mediated addition of centriolar microtubules

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Video 1
Wild-type embryo coexpressing GFP:γ-tubulin and GFP:histone. Wild-type embryo coexpressing GFP:γ-tubulin and GFP:histone imaged on a spinning disk confocal microscope. One 3D z stack (11 sections at 1-μm intervals) of confocal GFP images as well as a single central plane wide-field DIC image were acquired every 20 s. Each GFP frame is a maximal-intensity projection of the z stack from that time point. The GFP and DIC frames were montaged and are played back at 6 frames/s (i.e., 120× real time). The sequence begins during meiosis I and continues until the end of the first mitosis. Times are in min:s relative to the beginning of the video.