Krzewski et al. 10.1073/pnas.0711593105.
Fig. 5. (A) Localization of lytic granules and endogenous WIP. YTS cells, unconjugated or conjugated by mixing for 10 min at 37°C with 721.221 target cells, were stained with anti-WIP polyclonal antibody, followed by AlexaFluor 488-conjugated anti-goat secondary antibody (red) and AlexaFluor 647-conjugated anti-perforin monoclonal antibody (green). (Scale bar: 5 mm.) (B) WASp levels in different WIP transfectants. Postnuclear lysates from YTS cells, either untransfected (lane 1) or transfected with empty RNAi vector (lane 2), WIP RNAi (lane 3), or FLAG-WIP (lane 4) were resolved on a NuPage gel, followed by transfer and immunoblotting with anti-WIP, anti-WASp, and anti-actin (loading control) antibodies. The upper band in the last lane of the anti-WIP immunoblot corresponds to FLAG-WIP.