Fig. 6. Replacement of IL-12 fails to prolong the survival of Myd88^-/- mice infected with T. gondii. (A) Myd88^-/- mice were infected with T. gondii and given IL-12 or PBS daily, and survival was monitored. Infected Myd88^+/+ littermates given PBS were followed in parallel to control for parasite virulence. Data are pooled from two experiments (n = 8 mice each cohort). (B and C) CD4⁺ T cells isolated from mice on day 7 of infection were cultured in a 1:2 ratio with T cell-depleted splenocytes from uninfected B6 mice in the presence or absence of STAg. After 48 h, supernatants were collected for ELISA. Filled bar, STAg stimulated; open bar, unstimulated. Data represent two experiments (two to three mice per condition).

Fig. 7. TMyd88^-/- chimeric mice succumb to a lethal dose of LPS similar to wild-type mice. Mice from each group were injected with 1 mg LPS and monitored every 12 h for morbidity and mortality. Data are pooled from two experiments (n = 8 mice each cohort).

Fig. 8. High-dose IL-1Ra rescues mice from lethal challenge with LPS. Various doses of IL-1Ra (anakinra; Amgen) up to 15 mg (»500 mg/kg) were given 4 h before a lethal dose of LPS (0.6 mg, »40 mg/kg) and then every 24 h. Survival followed for 72 h. Results represent pooled data from three experiments.