Mesenchymal cell targeting by TNF as a common pathogenic principle in chronic inflammatory joint and intestinal diseases -- Data Supplement - JEM--Armaka et al. -- The Journal of Experimental Medicine

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Figure S2. MMP3 and MMP9 expression in SFs and IMFs from 4-wk-old WT and Tnf^ΔARE mice. (A) Immunofluorescent detection of MMP3 and MMP9 (Santa Cruz Biotechnology, Inc.) in SF cultures derived from 4-wk-old WT and Tnf^ΔARE/+ mice. WT SFs were stimulated with 20 ng/ml TNF for 8 h. (B) Immunofluorescent detection of MMP3 and MMP9 expression in IMF cultures derived from 4-wk-old WT and Tnf^ΔARE/+ mice. WT IMFs were stimulated with 20 ng/ml TNF for 24 h. Bars, 20 μm. (C) FITC-conjugated SMA and CD90 expression in WT and Tnf^ΔARE/+ SF cultures under serum-starved conditions. Isotype control (red shading), WT (black line), and Tnf^ΔARE/+ (green line) are shown.